We developed a biosensor that is capable for simultaneous surface plasmon resonance (SPR) sensing and hyperspectral fluorescence analysis in this paper. A symmetrical metal-dielectric slab scheme is employed for the excitation of coupled plasmon waveguide resonance (CPWR) in the present work. Resonance between surface plasmon mode and the guided waveguide mode generates narrower full width half-maximum of the reflective curves which leads to increased precision for the determination of refractive index over conventional SPR sensors. In addition, CPWR also offers longer surface propagation depths and higher surface electric field strengths that enable the excitation of fluorescence with hyperspectral technique to maintain an appreciable signal-to-noise ratio. The refractive index information obtained from SPR sensing and the chemical properties obtained through hyperspectral fluorescence analysis confirm each other to exclude false-positive or false-negative cases. The sensor provides a comprehensive understanding of the biological events on the sensor chips.

We propose a surface plasmon resonance (SPR) sensor based on phase modulation and polarization interferometry, both of which provide a refractive index (RI) resolution of the same order as that of SPR sensors of the phase type. And it has a wide dynamic range and insensitivity of RI resolution to the thickness of metal films as that of the intensity type SPR sensors. In this letter, we choose electro-optic (EO) phase modulation instead of the angle modulation. We demonstrate theoretically that with the EO phase modulation, our sensor could provide a better RI resolution.

表面等离子体共振(SPR)传感技术具有很高的折射率分辨率。但是其折射率分辨率对金属膜厚度非常敏感，影响了SPR传感器的适用性。使用一种叫做“偏振干涉”的技术，来减小光谱型SPR传感器的折射率对金膜厚度的敏感性。实验结果表面，偏振干涉能够降低SPR光谱的最小值，提高使用非最佳金属膜的SPR传感器的折射率分辨率。金膜厚度在28.16~54.38 nm范围内变化时，系统的折射率分辨率达到3.9×10-7~8.1×10-7RIU(折射率单位)。

介绍了基于光纤迈克尔孙干涉仪的三维谱域光学相干层析(OCT)系统。系统探测器部分为自行研制的高速高分辨率光谱仪,其光谱分辨率高达0.05 nm,使OCT成像探测深度范围达3.4 mm。系统信噪比(SNR)测量值为51 dB,纵向分辨率8.5 μm。系统成像速率达10×103 line /s,通过二维扫描和三维重建,可达到2 s采集一幅三维OCT图像的速度。借助三维相干层析成像技术,可以对眼底视网膜等生物组织进行更加直观和精确的显示。